COMMENT ON OHYUMI'S NITRO BLUE TETRAZOLIUM METHOD FOR THE ALLEGED HISTOCHEMICAL DEMONSTRATION OF RIBOSE 5-PHOSPHATE ISOMERASE

Inhibition of ribose-5-phosphate isomerase by 4-phosphoerythronate.

Hoping to exploit the special affinity of enzymes for unstable intermediates in substrate transformation, we have determined the effectiveness of possible analogs of ene-diolate intermediates as inhibitors of spinach ribose-5-phosphate isomerase. 4-Phosphoerythronic acid was found to be a very strong competitive inhibitor, with a Ki value almost 3 orders of magnitude lower than the Km value of ...

Nitro blue tetrazolium test in children with kwashiorkor with a comment on the use of latex particles in the test.

The nitro blue tetrazolium test was applied on neutrophils from children with kwashiorkor and from control children. The percentage of formazan cells formed in the kwashiorkor patients was significantly less than in the control group in spite of the presence of associated infections in the former. A direct correlation exists between the percentage of formazan cell formation and total serum prot...

Genetic studies on ribose 5-phosphate isomerase mutants of Escherichia coli K-12.

A gene for the constitutive ribosephosphate isomerase (rpiA) is highly cotransducible with serA at 56.2 min on the genetic linkage map of Escherichia coli K-12. Suppression of ribosephosphate isomerase A-negative mutants can occur by a regulator gene mutation permitting constitutive synthesis of the normally inducible ribosephosphate isomerase B.

Technique for the performance of the nitro-blue tetrazolium (NBT) test.

Disclosing the essentiality of ribose-5-phosphate isomerase B in Trypanosomatids

Ribose-5-phosphate isomerase (RPI) belongs to the non-oxidative branch of the pentose phosphate pathway, catalysing the inter-conversion of D-ribose-5-phosphate and D-ribulose-5-phosphate. Trypanosomatids encode a type B RPI, whereas humans have a structurally unrelated type A, making RPIB worthy of exploration as a potential drug target. Null mutant generation in Leishmania infantum was only p...